In the same frame-story format, Hader and Samberg apologize to Lorne Michaels for the original Laser Cats! claiming to understand where they went wrong: not enough politics. They then introduce Laser Cats! 2, based on the same premise, now set in the Iraq War which has gone nuclear and caused the feline mutations. This time, Dr. Scientist (Jake Gyllenhaal) has stolen the cure that turns Laser Cats back into regular cats. Jorma Taccone appears in the opening credits for Laser Cats! 2 (Which is the same as Laser Cats!), and Fred Armisen and Amy Poehler are the scientists who created the cure.

Samberg and Hader interrupt Lorne Michaels' dinner with Christopher Dodd (appearing as himself) to attempt to pitch Laser Cats! again, this time with a scene enhanced by 3D glasses. The plot revolves around a ban on all laser cats and a battle against the corrupt Mayor Top-Hat (Thompson). Christopher Walken appears as a general.

A "half human, half Laser Cat, half RoboCop" named Cyber-Face (Kenan Thompson) is presented to a group of scientists by their colleague (Will Forte) but quickly goes haywire. Admiral Spaceship (Samberg) and Nitro (Hader) intervene to put an end to its killing spree, and are shocked to find out that Cyber-Face is Admiral Spaceship's estranged father (Martin) right before he is gunned down by police officers.

Samberg and Hader once again try to persuade Lorne Michaels with another Laser Cats! movie with some "help" from James Cameron (appearing as himself).

Despite Lorne Michaels' objections, Hader and Samberg show him the latest Laser Cats short – this time directed by Steven Spielberg (appearing as both himself and a "Hitchcockian" cameo in the short), which contains many references to his films, including E.T. the Extra-Terrestrial, Close Encounters of the Third Kind, and Raiders of the Lost Ark. Host Josh Brolin appears as a "Space Nazi."

The Lonely Island celebrate their 100th Digital Short by revisiting many digital short favorite characters, clips from previous Digital Shorts, and fresh footage from numerous celebrities.